Ware, Bennie R. Department of Chemistry, Syracuse University, Syracuse, New York.
Lunte, Susan M. Center for Bioanalytical Research, University of Kansas, Lawrence, Kansas.
Gardiner, Kathleen Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado.
Last reviewed:March 2019
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- Gel Techniques
- Laser Applications
- Capillary Electrophoresis
- Capillary zone electrophoresis
- Capillary gel electrophoresis
- Alternating-Field Electrophoresis
- Links to Primary Literature
- Additional Readings
The migration of electrically charged particles in solution or suspension in the presence of an applied electric field. Each particle moves toward the electrode of opposite electrical polarity. For a given set of solution conditions, the velocity with which a particle moves divided by the magnitude of the electric field is a characteristic number called the electrophoretic mobility. The electrophoretic mobility is directly proportional to the magnitude of the charge on the particle, and is inversely proportional to the size of the particle. An electrophoresis experiment may be either analytical, in which case the objective is to measure the magnitude of the electrophoretic mobility, or preparative, in which case the objective is to separate various species which differ in their electrophoretic mobilities under the experimental solution conditions (Fig. 1).
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